Journal: Chemistry, an Asian Journal

Article Title: Advances in Small Molecule Inhibitors Targeting the Bacterial Lipoprotein Transport System (Lol) in Gram‐Negative Bacteria

doi: 10.1002/asia.202500350

Figure Lengend Snippet: Cryo‐EM structure of the E. coli LolCDE complex (PDB: 7ARI). LolC, LolD, and LolE are depicted in orange, magenta, and cyan, respectively.

Article Snippet: To elucidate the MoA, resistant mutants of E. coli ATCC 25922 ∆tolC were generated against compound 4 .

Techniques: Cryo-EM Sample Prep

Journal: Chemistry, an Asian Journal

Article Title: Advances in Small Molecule Inhibitors Targeting the Bacterial Lipoprotein Transport System (Lol) in Gram‐Negative Bacteria

doi: 10.1002/asia.202500350

Figure Lengend Snippet: (A) Structure of LolB (PDB: 1IWM) highlighting five conserved tryptophan residues represented as sticks. (B) Based on the LolAB complex structure (PDB: 8ORN) from Xanthomonas campestris pv. campestris, structural alignment with E. coli LolA (PDB: 7Z6W) and LolB (PDB: 1IWM) provides insights into their interaction. In the LolA–LolB complex, the conformation of the mLolB loop (66Q‐S70), highlighted in magenta, is expected to influence the precise association between LolA and LolB, potentially modulating lipoprotein transfer efficiency and specificity.

Article Snippet: To elucidate the MoA, resistant mutants of E. coli ATCC 25922 ∆tolC were generated against compound 4 .

Techniques:

Journal: Chemistry, an Asian Journal

Article Title: Advances in Small Molecule Inhibitors Targeting the Bacterial Lipoprotein Transport System (Lol) in Gram‐Negative Bacteria

doi: 10.1002/asia.202500350

Figure Lengend Snippet: Chemical structure of MAC13243 ( 9a ) and its SAR exploration. MICs were determined according to the NCCLS methods in E. coli MG1655.

Article Snippet: To elucidate the MoA, resistant mutants of E. coli ATCC 25922 ∆tolC were generated against compound 4 .

Techniques:

Journal: Chemistry, an Asian Journal

Article Title: Advances in Small Molecule Inhibitors Targeting the Bacterial Lipoprotein Transport System (Lol) in Gram‐Negative Bacteria

doi: 10.1002/asia.202500350

Figure Lengend Snippet: Hydrolysis of MAC13243 and discovery of isothiourea compounds. MICs in (B) and (C) were determined according to the NCCLS methods in E. coli MG1655.

Article Snippet: To elucidate the MoA, resistant mutants of E. coli ATCC 25922 ∆tolC were generated against compound 4 .

Techniques:

Journal: Journal of Bacteriology

Article Title: Genetic Evidence for the Involvement of the S-Layer Protein Gene sap and the Sporulation Genes spo0A , spo0B , and spo0F in Phage AP50c Infection of Bacillus anthracis

doi: 10.1128/JB.00739-13

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: It is temperature sensitive for replication and contains a chloramphenicol resistance gene for Gram-positive organisms, as well as a gene encoding the C9 variant of the Himar1 transposase and an erythromycin resistance gene, located between two inverted repeats that are transposed by the transposase. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid a Description Source or reference Strains Escherichia coli GM119 dam dcm mutant 36 C2925 dam dcm mutant New England Biolabs B. anthracis 34F 2 Sterne/pXO1 + /pXO2 − P. C. Hanna lab 7702 Sterne/pXO1 + /pXO2 − T. M. Koehler lab JB220 7702 ΔBAS2245 This study BAP350 7702 Δ csaB This study BA749 7702 ΔBAS0566 This study BA750 7702 Δ sap This study BA751 7702 Δ eag This study BA752 7702 ΔBAS1792 This study BA753 7702 ΔBAS3559 This study BA754 7702 Δ spo0A This study BA755 7702 Δ spo0F This study DP-B-5747 JB220 Δ spo0B This study B. cereus RS110 ATCC 4342 60 RS112 RTS100 ( B. anthracis -like) RS415 ATCC 25621 61 RS416 ATCC 43881 62 RS417 ATCC 14893 63 RS431 ATCC 27877 64 RS432 ATCC 7064 (NRS201) 60 RS436 B. cereus transition state strain CDC13100 47 RS437 B. cereus transition state strain CDC13140 47 RS438 B. cereus transition state strain CDC32805 47 RS710 B. cereus var.

Techniques: Plasmid Preparation, Mutagenesis, Expressing

Journal: Bioscience Reports

Article Title: Regulation of 5-oxo-ETE synthesis by nitric oxide in human polymorphonuclear leucocytes upon their interaction with zymosan and Salmonella typhimurium

doi: 10.1042/BSR20130136

Figure Lengend Snippet: ( A , B ) PMNLs (2×10 7 ) were incubated for 30 min at 37°C without additives or with 1 μM of different LPS forms or PMA (1–5 nM, as indicated), and then 2 mg/ml of zymosan was added for 15 min ( A ) or 5×10 8 S. typhimurium was added for 20 min ( B ). OZ or OS was added simultaneously with DEA NONOate (final concentration, 500 μM). The results represent the means±S.D. of four independent experiments, * P <0.05 and ** P <0.01, compared with the corresponding controls without PMA or LPS. ( C , D ) PMNLs (2×10 7 ) were incubated for 30 min at 37°C with 2 nM PMA, and then either 2 mg/ml OZ was added for 15 min ( C ) or 5×10 8 OS was added for 20 min ( D ). OZ or OS was added simultaneously with DEA NONOate at the indicated final concentration of NONOate (0.1–1 mM). The results represent the means±S.D. of four independent experiments, * P <0.05 and ** P <0.01, compared with the corresponding data without NO donor. ( E , F ) Time course of the 5-LOX reaction in PMNLs. PMNLs were incubated with 2 nM PMA for 30 min and then exposed to NO_OZ ( E ) or NO_OS ( F ) in a final concentration of 500 μM NONOate. The products generated by 5-LOX were extracted from the medium and separated using HPLC. The results are means±S.D. of three independent experiments performed in duplicate.

Article Snippet: HBSS (Hanks balanced salt solution) with calcium and magnesium but without phenol red and sodium hydrogen carbonate, Dulbecco's PBS with magnesium but without calcium, Dnp-Cl (1-chloro-2,4-dinitrobenzene), diamide and LPSs from Salmonella enterica serovar Typhimurium (smooth form derived from the strain ATCC 7823, Ra mutant TV119 and Re mutant SL1181) were from Sigma-Aldrich.

Techniques: Incubation, Concentration Assay, Generated